Optical Spectroscopy and Chemical Imaging (OSCI)
Sequence dependence of electron-induced DNA strand
Sequence dependence of electron-induced DNA strand breakage revealed by DNA nanoarrays
Adrian Keller;Jenny Rackwitz;Emilie Cauet;Jacques Lievin;Thomas Korzdorfer;Alexandru Rotaru;Kurt V. Gothelf;Flemming Besenbacher;Ilko Bald
The electronic structure of DNA is determined by its nucleotide sequence, which is for instance exploited in molecular electronics. Here we demonstrate that also the DNA strand breakage induced by low-energy electrons (18 eV) depends on the nucleotide sequence. To determine the absolute cross sections for electron induced single strand breaks in specific 13 mer oligonucleotides we used atomic force microscopy analysis of DNA origami based DNA nanoarrays. We investigated the DNA sequences 5'-TT(XYX)3TT with X = A, G, C and Y = T, BrU 5-bromouracil and found absolute strand break cross sections between 2.66 . 10(-14) cm(2) and 7.06 . 10(-14) cm(2). The highest cross section was found for 5'-TT(ATA)3TT and 5'-TT(ABrUA)3TT, respectively. BrU is a radiosensitizer, which was discussed to be used in cancer radiation therapy. The replacement of T by BrU into the investigated DNA sequences leads to a slight increase of the absolute strand break cross sections resulting in sequence-dependent enhancement factors between 1.14 and 1.66. Nevertheless, the variation of strand break cross sections due to the specific nucleotide sequence is considerably higher. Thus, the present results suggest the development of targeted radiosensitizers for cancer radiation therapy.


Journal Article
Scientific reports