Publications

Optical Spectroscopy and Chemical Imaging (OSCI)
Resonant formation of strand breaks in sensitized
Resonant formation of strand breaks in sensitized oligonucleotides induced by low-energy electrons (0.5 - 9.0 eV)
2017
R. Schürmann, T. Tsering, K. Tanzer, S. Denifl, S. V. K. Kumar, I. Bald
Halogenated nucleobases are used as radiosensitizers in cancer radiation therapy, enhancing the reactivity of DNA to secondary low‐energy electrons (LEEs). LEEs induce DNA strand breaks at specific energies (resonances) by dissociative electron attachment (DEA). Although halogenated nucleobases show intense DEA resonances at various electron energies in the gas phase, it is inherently difficult to investigate the influence of halogenated nucleobases on the actual DNA strand breakage over the broad range of electron energies at which DEA can take place (<12 eV). By using DNA origami nanostructures, we determined the energy dependence of the strand break cross‐section for oligonucleotides modified with 8‐bromoadenine (8BrA). These results were evaluated against DEA measurements with isolated 8BrA in the gas phase. Contrary to expectations, the major contribution to strand breaks is from resonances at around 7 eV while resonances at very low energy (<2 eV) have little influence on strand breaks.

Details

Journal Article
Angewandte Chemie
56
36
10952-10955
10.1002/anie.201705504

Download