Publications

Optical Spectroscopy and Chemical Imaging (OSCI)
Ion-selective formation of a guanine quadruplex on
Ion-selective formation of a guanine quadruplex on DNA origami structures
2015
Lydia Olejko;Piotr J. Cywinski;Ilko Bald
DNA origami nanostructures are a versatile tool that can be used to arrange functionalities with high local control to study molecular processes at a single-molecule level. Here, we demonstrate that DNA origami substrates can be used to suppress the formation of specific guanine (G) quadruplex structures from telomeric DNA. The folding of telomeres into G-quadruplex structures in the presence of monovalent cations (e.g. Na(+) and K(+)) is currently used for the detection of K(+) ions, however, with insufficient selectivity towards Na(+). By means of FRET between two suitable dyes attached to the 3'- and 5'-ends of telomeric DNA we demonstrate that the formation of G-quadruplexes on DNA origami templates in the presence of sodium ions is suppressed due to steric hindrance. Hence, telomeric DNA attached to DNA origami structures represents a highly sensitive and selective detection tool for potassium ions even in the presence of high concentrations of sodium ions.

Details

Journal Article
Angewandte Chemie (International ed. in English)
54
2
673--677
1433-7851
Olejko.2015
10.1002/anie.201409278

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